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Thermoresponsive polymers in chromatography
Thermoresponsive polymers can be used as stationary phase in liquid chromatography.〔Irene Tan, Farnoosh Roohi, Maria-Magdalena Titirici, ''Thermoresponsive polymers in liquid chromatography'', Analytical Methods, 2012, Volume 4, pages 34-43.〕 Here, the polarity of the stationary phase can be varied by temperature changes, altering the power of separation without changing the column or solvent composition. Thermally related benefits of gas chromatography can now be applied to classes of compounds that are restricted to liquid chromatography due to their thermolability. In place of solvent gradient elution, thermoresponsive polymers allow the use of temperature gradients under purely aqueous isocratic conditions. The versatility of the system is controlled not only through changing temperature, but through the addition of modifying moieties that allow for a choice of enhanced hydrophobic interaction, or by introducing the prospect of electrostatic interaction. These developments have already introduced major improvements to the fields of hydrophobic interaction chromatography, size exclusion chromatography, ion exchange chromatography, and affinity chromatography separations as well as pseudo-solid phase extractions ("pseudo" because of phase transitions).
==Hydrophobic interaction chromatography==
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